US Patent 5,057,420
- CLAIM 1. A process to produce viable bovine embryos through nuclear transplantation comprising the following steps:
- (a) collecting recipient oocytes from donor cows;
- (b) collecting embryos greater than 32 cells;
- (c) removing a portion of the ooplasm from the recipient oocyte;
- (d) separating the embryo cells into single donor cells for nuclear transplantation;
- (e) inserting said donor cell into the recipient oocyte;
- (f) electrofusing the oocyte and donor cell to form an embryo; and
- (g) culturing said embryo.