US Patent 4,994,384
- CLAIM 15. A method for producing cloned bovine embryos comprising:
- (a) isolating a nucleus, bounded by a membrane, from a single cell of a two to thirty-two cell donor bovine embryo;
- (b) enucleating a recipient oocyte;
- (c) introducing the donor membrane-bounded nucleus into the perivitelline space of the enucleated recipient oocyte;
- (d) electrically inducing cell fusion between the membrane bounding the donor nucleus and the enucleated recipient oocyte;
- (e) culturing in vitro the electrically induced fused embryo obtained in step (d); and
- (f) transferring the cultured embryo of step (e) to the oviduct of a recipient maternal animal.